We offer ICC-CLASS (Isotopically-Coded Cleavable CrossLinking Analysis Software Suite) for data processing of experiments with isotopically-coded cleavable crosslinkers.

  
ICC-CLASS (Isotopically-Coded Cleavable CrossLinking Analysis Software Suite) is a collection of software tools for analysis of mass spectrometric data from experiments using isotopically-coded cleavable crosslinking reagents.

Main functionalities of ICC-CLASS are 1) search for doublets of signals corresponding to isotopic coding of the crosslinks (DX program); 2) crosslinks type identification through the matching of cleaved crosslinks masses to the uncleaved ones (DXDX program); 3) assignment of the uncleaved crosslinks based on both isotopically-coded and non-coded MSMS crosslinks fragments (DXMSMS program).

Software products are not for redistribution and are for research use only.
 
Trial versions of windows applications can be installed through links below. Programs require .NET Framework from Microsoft to be installed on the computer. When application is installed, a shortcut will be added to the Start Menu, and the application can be uninstalled via Add/Remove Programs.
Program: Description:
DX VB Search for doublets of signals in mass list text files from MS spectra.
DXDX VB Matching the cleaved to the uncleaved crosslinks doublets in multiple pairs of MS spectra.
DXMSMS VB Crosslinks assignment based on MSMS data.
 
Other useful programs for processing of crosslinking MS data as windows applications executable modules.
Program: Description:
DXMultipletFinder Search for multiplets of signals in mass list text files from MS spectra.
DXMassListFilter Removal of duplicates from doublets mass list text files of MS spectra of chromatographic fractions.
DXDX ICCL ICNTM Identification of the inter-peptide crosslinks by comparing ICCL and ICCL+ICNTM runs.
Peptides CL Prediction of the masses of the MSMS fragments of the crosslink from two peptides.
ICCL MSMS Analysis of the MSMS spectra of the CID-cleavable isotopically-coded crosslinks.
DX Search for doublets of signals in mass list text files from MS spectra.
DXDX Matching the cleaved to the uncleaved crosslinks doublets in multiple pairs of MS spectra.
DXMSMS Crosslinks assignment based on MSMS data.
 
Standalone executables of ICC-CLASS.
Program: Control file: Description: Instructions file:
DX.exe DX_Controls.txt Search for doublets of signals in the single mass list text file.DX_Read_Me.txt
DXSet.exe DXSet_Controls.txt Search for doublets of signals in the multiple mass list text files.DXSet_Read_Me.txt
DXDX.exe DXDX_Controls.txt Matching the cleaved to the uncleaved crosslinks doublets in a single pair of uncleaved and cleaved spectra.DXDX_Read_Me.txt
DXDXSet.exe DXDXSet_Controls.txt Matching the cleaved to the uncleaved crosslinks doublets in multiple pairs of uncleaved and cleaved spectra.DXDXSet_Read_Me.txt
DXMSMS.exe DXMSMS_Controls.txt Crosslinks assignment based on MSMS data.DXMSMS_Read_Me.txt
DXMSMS_Mass.txt
DXMSMS_Fragments.txt
DXMSMS_DX_Fragments.txt
DXMSMS_Cleavage_Products.txt
DXMSMS_Sequences.txt